Author: c001os

A team of European scientists has found a key pig gene for the replication of African Swine Fever (ASF) virus. Researchers from the Friedrich-Loeffler-Institute (FLI) in Germany and the Roslin Institute of the University of Edinburgh, Scotland, recently conducted this piece of research, which aimed to find out which pig genes are needed for the ASF virus (ASFv) to replicate. The study describing their findings was published in August 2023 in the peer-reviewed scientific journal Scientific Reports and shows that there is a gene from the pig’s immune system that is key to this process. This provides important new insights into the biology of the ASFv that could form the basis for future research approaches. ASFv has a large DNA genome from which more than 160 viral proteins are produced in infected cells. Little is known about the functions of many of these viral proteins. It is also unclear which cellular proteins are used by the ASF virus to enter the host cell. To identify host proteins important for ASFv, scientists at the Roslin Institute provided a CRISPR/Cas9 expression library as a molecular tool that allowed their colleagues Friedrich-Loeffler-Institute to knock out all known genes in the pig genome individually in vitro and test the resulting cell cultures for susceptibility to ASF virus infection. That led to the identification of several genes of the major histocompatibility complex II (MHC II) as relevant factors for the reproductive capacity of ASFv, the news release explained. In particular, the MHC II receptor protein SLA-DM was shown to be required for efficient ASFv infection. Therefore, the research team concluded, SLA-DM may be a suitable target protein for the development of effective therapeutics against ASF or ASFv resistant pig breeds.

More than 70% of the body’s immune cells are located into the gut, which means there is a close connection between intestinal microbiota and the immune system. The sow’s microbiota influences the piglet’s immune system development and, consequently, their growth and survival. At birth and during early life, the piglets are highly dependent on the maternal passively derived immunity for the survival. The transfer of immunity initially occurs through colostrum. Antibodies are big proteins, and only get into the piglet’s blood stream in the first hours of life before the gut closure. Immunoglobulins from the mother, including immunoglobulin G (IgG) can be absorbed through the colostrum. The probiotic yeast Saccharomyces cerevisiae boulardii CNCM-I-1079 included into the sows’ diet, has shown positive results on the IgG colostrum concentration. Recent studies have shown that animals raised on artificial milk formula rather than maternal milk have a higher susceptibility to disease, also linked to a reduced intestinal microbiota diversity. Cytokines and chemokines are making connections between the different organs to coordinate the immune response. They are biomarker peptides critical for humoral and cell mediated immunity. Cytokine gene expression in the lungs of piglets was influenced by the treatment of sows. The lower expression of cytokines in the lungs of the piglets born and nursed from supplemented sows suggests a reduced inflammatory reaction compared to the piglets from non-supplemented sows. The immune transfer through colostrum, modulation inflammatory response, etc. translated to better performance of the piglets after weaning, reflecting maternal imprinting. Probiotics, such as Saccharomyces cerevisiae boulardii CNCM-I-1079, are positively affecting the gut microbiota from the sow to her piglets and driving their immune system to a more mature one and are interesting tools to support piglet quality and reduce antibiotic use.

Softer consumption trends in early 2023 are prompting caution in the global pork industry as it struggles to adjust to a moving target, according to Rabobank’s latest quarterly pork report. Weaker economic growth is starting to have an impact on global pork consumption. Although the worst of the inflationary effects appear to be in the past, the lagged impact on consumption is likely to be felt throughout 2023. In a slowing economy, pork remains well-positioned, as demand for the protein is historically less income-sensitive than more expensive proteins like beef and premium seafood. “Nevertheless, we see persistently high retail prices limiting consumption of all proteins. Consumers continue to conserve capital by shifting everyday purchases to lower-value protein options, switching channels, and moving to smaller pack sizes,” says Christine McCracken, senior analyst – animal protein at Rabobank. Moreover, industry optimism in 2022 after a notable upward shift in pork consumption (and prices) in some markets and expectations of a 2023 recovery of pandemic-restricted consumption in others contributed to planned supply growth in 2023. That growth will take time to curb. “Slowing supply in Europe will help balance the industry, yet high costs of production and limited consumer support will require a more conservative approach to production to stabilize margins,” McCracken said. Although a modest improvement in production costs is expected in 2023, local conditions will vary, and risk management will remain critical to success. Global feed stocks are at historically low levels, and availability remains tight. A disappointing Argentine harvest will partially offset Brazil’s record 2023 soybean and safrinha corn crops, leaving the market to focus on import needs, Black Sea grain availability, and the successful planting of a new crop in the Northern Hemisphere. “Rabobank expects the small global cushion in grain and oilseed stocks to drive additional feed cost volatility in 2023,” McCracken said.